SIZE-FINITE SCALING

 

In vitro models constituted by human cells have been developed for the extrapolation of biological parameters to in vivo contexts....but how to quantitatively define if an organ in a dish can infer pathophysiological features of its in vivo counterpart? 

To establish design criteria for such models and revealing their extent of similarity with living organisms as a benchmark of physiological relevance, we leverage on allometric scaling laws, which pertains to all living organisms. 

KL is one of the most well-known allometric relationships in biology. It states that the basal metabolic rate (BMR) of an organism scales with its body mass, M, according to a universal power-law BMR = aMα

We are focusing on understanding how scaling features emerge in cell cultures, to establish design criteria for such models and revealing their extent of similarity with living organisms as a benchmark of physiological relevance.

We are focusing on understanding how scaling features emerge in cell cultures, to establish design criteria for such models and revealing their extent of similarity with living organisms as a benchmark of physiological relevance.

 

Since fluctuations are ubiquitous in nature and intrinsic features of biological systems, we are incorporating also fluctuations and variability.

We have already shown, via a computational pipeline, how to identify the experimental size range of cell cultures with quantifiable similitudes in terms of fluctuations and metabolic scaling with living organisms.
In parallel, experiments are now ongoing on in vitro constructs ad increasing complexity integrating high-precision oxygen sensors, algorithms for metabolic parameters identification and mass measurements.

   

 

The experimental and computational framework developed by our group underpins the sound design of statistically meaningful in vitro models possessing translational potential, with impacts in many areas of biomedical science and ecology.

 

 

   Our publications:

1. Ahluwalia, Arti, "Allometric Scaling in vitro". Scientific Reports, 7.1 (2017):1-7

2. Magliaro, Chiara, Andrea Rinaldo, and Arti Ahluwalia. "Allometric Scaling of physiologically-relevant organoids." Scientific reports 9.1 (2019): 1-8.

3. Botte, E., Biagini, F., Magliaro, C., Rinaldo, A., Maritan, A., & Ahluwalia, A. (2021). Scaling of joint mass and metabolism fluctuations in in silico cell-laden spheroids. Proceedings of the National Academy of Sciences, 118(38).

 

 

 

    Contact Info:

Arti Ahluwalia

Chiara Magliaro

Ermes Botte