SENSEI is a Human Brain Project Partnering Project. Its objectives are to accurately quantify neuronal morphology at tissue and molecular levels through the development of intelligent segmentation-based image processing algorithms and to improve the quality of neuronal imaging using new membrane probes for conventional and emerging super-resolution imaging technologies. Specifically, we will develop an algorithm for isolating single neurons or smaller neuronal structures from 3D datasets representing the intricacies of the brain micro-structure.