01787nas a2200205 4500008004100000022001400041245013800055210006900193520112600262653001901388100001701407700001701424700001401441700001501455700001301470700001701483700001501500700001801515856004801533 2013 eng d a1743-540400aIn vitro toxicological screening of nanoparticles on primary human endothelial cells and the role of flow in modulating cell response0 aIn vitro toxicological screening of nanoparticles on primary hum3 a
Abstract After passage through biological barriers, nanomaterials inevitably end up in contact with the vascular endothelium and physiological flow, and can induce cardiovascular damage. In this study the toxicity and sublethal effects of 6 nanoparticles, including 4 of industrial and biomedical importance, on human endothelial cells was investigated using different in vitro assays. The results show that all the particles investigated induce some level of damage to the cells and that silver particles were most toxic, followed by titanium dioxide. Furthermore endothelial cells were shown to be more susceptible when exposed to silver nanoparticles under flow conditions in a bioreactor. The study underlines that although simple in vitro tests are useful to screen compounds and to identify the type of effect induced on cells, they may not be sufficient to define safe exposure limits. Therefore, once initial toxicity screening has been conducted on nanomaterials, it is necessary to develop more physiologically relevant in vitro models to better understand how nanomaterials can impact on human health.
10aBioengineering1 aUcciferri, N1 aCollnot, E M1 aGaiser, B1 aTirella, A1 aStone, V1 aDomenici, C.1 aLehr, C -M1 aAhluwalia, A. uhttp://www.ncbi.nlm.nih.gov/pubmed/23909703