%0 Journal Article %J PLoS ONE %D 2018 %T Systemic and vascular inflammation in an in-vitro model of central obesity %A A. Ahluwalia %A A. Misto %A G. Vozzi %A C. Magliaro %A G. Mattei %A Marescotti MC %A AVOGARO, A %A Iori, E. %K Bioengineering %X

Metabolic disorders due to over-nutrition are a major global health problem, often associated with obesity and related morbidities. Obesity is peculiar to humans, as it is associated with lifestyle and diet, and so difficult to reproduce in animal models. Here we describe a model of human central adiposity based on a 3-tissue system consisting of a series of interconnected fluidic modules. Given the causal link between obesity and systemic inflammation, we focused primarily on pro-inflammatory markers, examining the similarities and differences between the 3-tissue model and evidence from human studies in the literature. When challenged with high levels of adiposity, the in-vitro system manifests cardiovascular stress through expression of E-selectin and von Willebrand factor as well as systemic inflammation (expressing IL-6 and MCP-1) as observed in humans. Interestingly, most of the responses are dependent on the synergic interaction between adiposity and the presence of multiple tissue types. The set-up has the potential to reduce animal experiments in obesity research and may help unravel specific cellular mechanisms which underlie tissue response to nutritional overload.

%B PLoS ONE %8 02/2018 %G eng %U http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0192824 %R https://doi.org/10.1371/journal.pone.0192824.s001 %0 Journal Article %J Biofabrication %D 2016 %T fabrication and perivascular implantation of bioactive scaffolds engineered with human adventitial progenitor cells for stimulation of arteriogenesis in peripheral ischemia %A Carrabba, M. %A C. De Maria %A A. Oikawa %A C. Reni %A I. Rodriguez-Arabaolaza %A H. Spencer %A S. Slater %A E. Avolio %A Z. Dang %A G. Spinetti %A MADEDDU, P. %A G. Vozzi %K Bioengineering %B Biofabrication %V 8 %8 03/24 %G eng %N 1:015020 %R 10.1088/1758-5090/8/1/015020 %0 Journal Article %J J Biomed Mater Res Part B. %D 2016 %T The influence of mesh topology in the abdominal wall repair process %A C. De Maria %A S. Burchielli %A C. Salvadori %A V. Santoro %A F. Montemurro %A G. Orsi %A G. Vozzi %K Bioengineering %B J Biomed Mater Res Part B. %8 08/2016 %G eng %U https://www.ncbi.nlm.nih.gov/pubmed/26097153 %R https://doi.org/10.1002/jbm.b.33468 %0 Book Section %B Nanobiomaterials in Soft Tissue Engineering - Applications of Nanobiomaterials %D 2016 %T Machine design for multimaterial processing %A C. De Maria %A De Acutis, A %A Carrabba, M. %A G. Criscenti %A G. Vozzi %B Nanobiomaterials in Soft Tissue Engineering - Applications of Nanobiomaterials %I Elsevier %G eng %0 Journal Article %J J Mech Behav Biomed Mater. %D 2016 %T Reconstruction of medial patello-femoral ligament: Comparison of two surgical techniques %A Criscenti, G. %A C. De Maria %A E. Sebastiani %A M. Tei %A G. Placella %A G. Vozzi %A Cerulli, G. %K Bioengineering %B J Mech Behav Biomed Mater. %P 272-278 %8 02/10 %G eng %N 59 %R 10.1016/j.jmbbm.2016.02.009 %0 Journal Article %J Biofabrication %D 2016 %T riphasic scaffolds for the regeneration of the bone-ligament interface %A G. Criscenti %A A. Longoni %A A. Di Luca %A C. De Maria %A Van Blitterswijk, C. %A G. Vozzi %A Moroni, L. %B Biofabrication %V 8 %G eng %N 1 %& 015009 %R 10.1088/1758-5090/8/1/015009 %0 Journal Article %J Computer methods in biomechanics and biomedical engineering %D 2015 %T ADMET: ADipocyte METabolism mathematical model %A Micheloni, A. %A G. Orsi %A C. De Maria %A G. Vozzi %K Bioengineering %B Computer methods in biomechanics and biomedical engineering %V 18(13) %P 1386 -1391 %G eng %0 Journal Article %J Current Molecular Biology Reports %D 2015 %T Biomimicking of the Breast Tumor Microenvironment %A M. Giussani %A C. De Maria %A M. Vasso %A F. Montemurro %A T. Triulzi %A E. Tagliabue %A C. Gelfi %A G. Vozzi %K Bioengineering %B Current Molecular Biology Reports %V 1 (2) %P 71-76 %G eng %0 Journal Article %J Computers and Chemical Engineering %D 2015 %T CFD modelling of a mixing chamber for the realisation of functionally graded scaffolds %A G. Mattei %A G. Vozzi %K Bioengineering %X

Biological tissues are characterised by spatially distributed gradients, intricately linked with functions. It widely accepted that ideal tissue engineered scaffolds should exhibit similar functional gradients to promote successful tissue regeneration. Focusing on bone, in previous work we proposed simple methods to obtain osteochondral functionally graded scaffolds (FGSs), starting from homogeneous suspensions of hydroxyapatite (HA) particles in gelatin solutions. With the main aim of developing an automated device to fabricate FGSs, this work is focused on designing a stirred tank to obtain homogeneous HA-gelatin suspensions. The HA particles transport within the gelatin solution was investigated through computational fluid dynamics (CFD) modelling. First, the steady-state flow field was solved for the continuous phase only. Then, it was used as a starting point for solving the multi-phase transient simulation. CFD results showed that the proposed tank geometry and setup allow for obtaining a homogeneous suspension of HA micro-particles within the gelatin solution.

%B Computers and Chemical Engineering %G eng %U http://www.sciencedirect.com/science/article/pii/S0098135415002823 %R 10.1016/j.compchemeng.2015.08.021 %0 Journal Article %J Current topics in medicinal chemistry %D 2015 %T Combining Inkjet Printing and Sol-Gel chemistry for Making pH-Sensitive surfaces %A G. Orsi %A C. De Maria %A F. Montemurro %A Chauhan, V. %A Aylott, J. %A G. Vozzi %K Bioengineering %B Current topics in medicinal chemistry %V 15(3) %P 271-278 %G eng %0 Journal Article %J Procedia Engineering %D 2015 %T Design and Validation of an Open-Hardware Print-Head forBioprinting Application %A C. De Maria %A L. Ferrari %A F. Montemurro %A G. Vozzi %A I. Guerrazzi %A Boland, T. %A G. Vozzi %K Bioengineering %B Procedia Engineering %V 110 %P 98-105 %G eng %0 Book Section %B Essential of 3D Biofabrication and Translation %D 2015 %T Indirect Rapid Prototyping for Tissue Engineering %A C. De Maria %A De Acutis, A %A G. Vozzi %K Bioengineering %B Essential of 3D Biofabrication and Translation %I Elsevier %G eng %0 Journal Article %J Sensors Journal %D 2015 %T Magnetic-Driven Pointing System: a Feasibility Study %A G. Russo %A C. De Maria %A Cerulli, G. %A G. Vozzi %K Bioengineering %B Sensors Journal %V 15 (2) %P 703 - 714 %8 02/2015 %G eng %0 Journal Article %J Journal of Biomedical Materials Research - Part B Applied Biomaterials %D 2015 %T Realisation and characterization of conductive hollow fibers for neuronal tissue engineering %A Gattazzo, F. %A C. De Maria %A Whulanza, Y. %A Taverni, G. %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Journal of Biomedical Materials Research - Part B Applied Biomaterials %V 103 %P 1107-1119 %G eng %0 Book Section %B Metodiche alternative alla sperimentazione animale %D 2015 %T Utilizzo di Phantom in oftalmologia %A G. Vozzi %A C. De Maria %A F. Montemurro %K Bioengineering %B Metodiche alternative alla sperimentazione animale %I Pàtron Editore %C Bologna %V 34 %G eng %6 pp.125-143 %0 Journal Article %J Computer methods in biomechanics and biomedical engineering %D 2014 %T ADMET: ADipocyte METabolism mathematical model %A Micheloni, A. %A G. Orsi %A C. De Maria %A G. Vozzi %K Bioengineering %B Computer methods in biomechanics and biomedical engineering %P 1–6 %G eng %0 Journal Article %J Journal of reconstructive microsurgery %D 2014 %T An Autologously Generated Platelet-Rich Plasma Suturable Membrane May Enhance Peripheral Nerve Regeneration after Neurorraphy in an Acute Injury Model of Sciatic Nerve Neurotmesis %A Giannessi, E. %A Coli, A. %A Stornelli, M. R. %A Miragliotta, V. %A Pirone, A. %A Lenzi, C. %A Burchielli, S. %A G. Vozzi %A C. De Maria %A Giorgetti, M. %K Bioengineering %B Journal of reconstructive microsurgery %G eng %0 Journal Article %J Cell biochemistry and biophysics %D 2014 %T Characterization of 3-Iodothyronamine In Vitro Dynamics by Mathematical Modeling %A G. Orsi %A Ghelardoni, S. %A Saba, A. %A R. Zucca %A G. Vozzi %B Cell biochemistry and biophysics %V 68 %P 37–47 %G eng %0 Journal Article %J Journal of Biomedical Materials Research Part A %D 2014 %T Collagen-gelatin-genipin-hydroxyapatite composite scaffolds colonized by human primary osteoblasts are suitable for bone tissue engineering applications: In vitro evidences %A G. Vozzi %A Corallo, C. %A Carta, S. %A Fortina, M. %A Gattazzo, F. %A Galletti, M. %A N. Giordano %B Journal of Biomedical Materials Research Part A %V 102 %P 1415–1421 %G eng %0 Journal Article %J IEEE Transactions of Biomedical Engineeirng %D 2014 %T CREPE: mathematical model for CRosstalking of Endothelial cells and hePatocytE metabolism %A Valvano, G %A G. Orsi %A M. A. Guzzardi %A Vozzi, F. %A G. Vozzi %K Bioengineering %B IEEE Transactions of Biomedical Engineeirng %V 61 %P 224 - 230 %8 01/2014 %G eng %N 1 %R 10.1109/TBME.2013.2272942 %0 Journal Article %J Rapid Prototyping Journal %D 2014 %T Development of a novel Micro-Ablation System to realise micrometric and well-defined hydrogel structures for Tissue Engineering applications %A C. De Maria %A Grassi, L. %A Vozzi, F. %A A. Ahluwalia %A G. Vozzi %A Campbell, R. I. %K Bioengineering %B Rapid Prototyping Journal %V 20 %G eng %0 Journal Article %J Journal of tissue engineering and regenerative medicine %D 2014 %T In vitro development of engineered muscle using a scaffold based on the pressure-activated microsyringe (PAM) technique %A D. Cei %A Malena, A. %A C. De Maria %A Loro, E. %A Sandri, F. %A Moro, G. %A Bettio, S. %A Vergani, L. %A G. Vozzi %B Journal of tissue engineering and regenerative medicine %G eng %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T In-silico model of cell metabolism in dynamic cell culture system %A Andreoni, C. %A G. Orsi %A C. De Maria %A F. Montemurro %A G. Vozzi %K Bioengineering %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 487–488 %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T Keratin based hydrogels for tissue engineering and regenerative medicine %A F. Montemurro %A Gattazzo, F. %A C. De Maria %A G. Vozzi %K Bioengineering %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 357–358 %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T Micro-patterned gelatin-genipin hydrogel for skeletal muscle tissue engineering %A Gattazzo, F. %A G. Orsi %A G. Vozzi %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 351–352 %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T Multimaterial and multiscale biofabrication for smart scaffolds %A C. De Maria %A Carrabba, M. %A G. Criscenti %A G. Orsi %A F. Montemurro %A G. Vozzi %K Bioengineering %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 470–470 %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T Nanoparticles doped sol-gel ink for inkjet printers %A G. Orsi %A C. De Maria %A F. Montemurro %A Chauhan, V. %A Aylott, J. %A G. Vozzi %K Bioengineering %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 197–198 %0 Journal Article %J Journal of Tissue Engineering and Regenerative Medicine %D 2014 %T A new 3D concentration gradient maker and its application in building hydrogels with a 3D stiffness gradient %A G. Orsi %A Fagnano, M. %A C. De Maria %A F. Montemurro %A G. Vozzi %K Bioengineering %B Journal of Tissue Engineering and Regenerative Medicine %G eng %0 Journal Article %J Journal of biomedical optics %D 2014 %T New eye phantom for ophthalmic surgery. %A Fogli, G. %A G. Orsi %A C. De Maria %A F. Montemurro %A Palla, M. %A Rizzo, S. %A G. Vozzi %K Bioengineering %B Journal of biomedical optics %V 19 %P 68001 %G eng %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T A novel concentration gradient bioreactor by PMMA laser micromachining %A G. Orsi %A C. De Maria %A F. Montemurro %A G. Vozzi %K Bioengineering %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 482–482 %0 Journal Article %J Journal of Biomedical Materials Research Part B: Applied Biomaterials %D 2014 %T Realisation and characterization of conductive hollow fibers for neuronal tissue engineering %A Gattazzo, F. %A C. De Maria %A Whulanza, Y. %A Taverni, G. %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Journal of Biomedical Materials Research Part B: Applied Biomaterials %G eng %0 Conference Paper %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %D 2014 %T Realization of a soft-MI electrospun scaffold for tissue engineering applications %A G. Criscenti %A Cerulli, G. %A Saris, D. %A Van Blitterswijk, C. %A G. Vozzi %A Fernandes, H. %A Moroni, L. %B JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE %I WILEY-BLACKWELL 111 RIVER ST, HOBOKEN 07030-5774, NJ USA %P 255–256 %0 Journal Article %J Tissue Engineering Part A %D 2014 %T Role of IGF1 and IGF1/VEGF on Human Mesenchymal Stromal Cells in Bone Healing: Two Sources and Two Fates %A Ferretti, C. %A G. Vozzi %A Falconi, M. %A Orciani, M. %A Gesi, M. %A Di Primio, R. %A Mattioli-Belmonte, M. %B Tissue Engineering Part A %G eng %0 Journal Article %J Smart Membranes and Sensors: Synthesis, Characterization, and Applications %D 2014 %T Smart Sensing Scaffolds %A C. De Maria %A Whulanza, Y. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B Smart Membranes and Sensors: Synthesis, Characterization, and Applications %P 337 %G eng %0 Journal Article %J MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS %D 2013 %T Bone scaffolds with homogeneous and discrete gradient mechanical properties %A Jelen, C. %A G. Mattei %A F. Montemurro %A C. De Maria %A Mattioli-Belmonte, M. %A G. Vozzi %K Bioengineering %B MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS %V 1 %P 28–36 %G eng %0 Journal Article %J Cell Biochem Biophys %D 2013 %T Characterization of 3-Iodothyronamine In Vitro Dynamics by Mathematical Modeling %A G. Orsi %A S. Ghelardoni %A A. Saba %A R. Zucca %A G. Vozzi %K Bioengineering %B Cell Biochem Biophys %G eng %R 10.1007/s12013-013-9680-y %0 Journal Article %J Nat Commun %D 2013 %T Collagen VI regulates satellite cell self-renewal and muscle regeneration %A Urciuolo, A %A M. Quarta %A V. Morbidoni %A Gattazzo, F %A S. Molon %A P. Grumati %A F. Montemurro %A F. S. Tedesco %A B. Blaauw %A G. Cossu %A G. Vozzi %A T. A. Rando %A Bonaldo, P %K Bioengineering %B Nat Commun %V 4 %G eng %R 10.1038/ncomms2964 %0 Journal Article %J J Biomed Mater Res A %D 2013 %T Collagen-gelatin-genipin-hydroxyapatite composite scaffolds colonized by human primary osteoblasts are suitable for bone tissue engineering applications: In vitro evidences %A G. Vozzi %A C. Corallo %A S. Carta %A M. Fortina %A Gattazzo, F %A M. Galletti %A N. Giordano %K Bioengineering %B J Biomed Mater Res A %8 06/2013 %G eng %0 Journal Article %J Journal of Biomedical Materials Research Part A %D 2013 %T Collagen‐gelatin‐genipin‐hydroxyapatite composite scaffolds colonized by human primary osteoblasts are suitable for bone tissue engineering applications: In vitro evidences %A G. Vozzi %A C. Corallo %A S. Carta %A M. Fortina %A Gattazzo, F %A M. Galletti %A N. Giordano %K Bioengineering %B Journal of Biomedical Materials Research Part A %G eng %R 10.1002/jbm.a.34823 %0 Book Section %B Approccio Integrato per la medicina rigenerativa - book of XXXII School of Bioengineering %D 2013 %T Da scaffold a tessuti: progettazione, realizzazione e caratterizzazione di architetture 3D per la medicina rigenerativa %A Tirella, A %A C. De Maria %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B Approccio Integrato per la medicina rigenerativa - book of XXXII School of Bioengineering %G eng %0 Journal Article %J Journal of nanoscience and nanotechnology %D 2013 %T Electrical and Mechanical Characterisation of single wall carbon nanotubes based composites for tissue engineering applications %A Whulanza, Y %A L. Vannozzi %A Vomero, M. %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Journal of nanoscience and nanotechnology %P 188 - 197 %8 01/2013 %G eng %0 Journal Article %J Polymers Advanced Technologies %D 2013 %T A new approach to fabricate agarose microstructures %A C. De Maria %A J. Rincon %A A.A. Duarte %A G. Vozzi %A Boland, T %K Bioengineering %B Polymers Advanced Technologies %G eng %R 10.1002/pat.3162 %0 Journal Article %J Journal of Apllied polymer science %D 2013 %T Pressure-activated microsyringe composite scaffold of poly(L-lactic acid) and carbon nanotubes for bone tissue engineering %A G. Vozzi %A C. Corallo %A C. Daraio %K Bioengineering %B Journal of Apllied polymer science %V 129 %P 528–536 %G eng %N 2 %0 Journal Article %D 2013 %T Silk Fibroin/Gelatin Blend Films Crosslinked with Enzymes for Biomedical Applications %A P. Taddei %A V. Chiono %A A. Anghileri %A G. Vozzi %A G. Freddi %A G. Ciardelli %K Bioengineering %G eng %R 10.1002/mabi.201300156 %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T 3D SOFT-MI: novel microfabrication method of 3D molecular imprinted structures %A Carrabba, M %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 132 - 133 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Characterization and modeling of mechanical behavior a discrete gradient structure %A Caputo, M %A G. Vozzi %A G. Mattei %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 130 - 131 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Design, characterization and modeling of microfabricated Soft-MI for ophthalmologic applications. %A La Vecchia, C %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 204 - 205 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Finite element model for PAM scaffolds %A Pimentel, R %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 74 - 75 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Fluid dynamics characterization of a microfluidic concentration gradient maker %A G. Orsi %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 152 - 153 %G eng %0 Book Section %B Hydrogels: Synthesis, Characterization and Applications %D 2012 %T Hydrogels with 3D Gradient of Mechanical Properties %A G. Orsi %A Carta, V %A G. Vozzi %K Bioengineering %B Hydrogels: Synthesis, Characterization and Applications %7 online edition %G eng %& Hydrogels with 3D Gradient of Mechanical Properties %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Hydrogels with 3D gradient of mechanical properties %A Carta, V %A G. Orsi %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 200 - 201 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Indirect m-fabrication using Pam2 system %A De Acutis, A %A C. De Maria %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 166 - 167 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Influence of substrate stiffness on behaviour of satellite cells %A Gattazzo, F %A Urciuolo, A %A Bonaldo, P %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 78 - 79 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Mathematical modelling of Endothelin-1 effect on hepatocytes, %A Valvano, G %A G. Orsi %A M. A. Guzzardi %A Vozzi, F. %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 205 - 206 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Natural bioactive matrix for cartilage tissue engineering %A F. Montemurro %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 161 - 162 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti, pp 341-342, Rome %D 2012 %T A novel approach to fabricate agarose microstructures, %A C. De Maria %A Boland, T %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti, pp 341-342, Rome %G eng %0 Journal Article %J MOLECULAR BIOTECHNOLOGY %D 2012 %T A Novel Method to Produce Immobilised Biomolecular Concentration Gradients to Study Cell Activities: Design and Modelling %A G. Vozzi %A Lenzi, T %A F. Montemurro %A Pardini, C %A Vaglini, F %A A. Ahluwalia %K Bioengineering %B MOLECULAR BIOTECHNOLOGY %V 50 %P 99-107 %G eng %R 10.1007/s12033-011-9411-9 %0 Journal Article %J Rapid Prototyping Journal %D 2012 %T The PAM2 system: a multilevel approach for fabrication of complex three-dimensional microstructures %A Tirella, A %A C. De Maria %A G. Criscenti %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B Rapid Prototyping Journal %V 18 %P 299–307 %G eng %R 10.1108/13552541211231725 %0 Journal Article %J Methods in Molecular Biology %D 2012 %T Rapid Prototyping Composite and Complex Scaffolds with PAM2 %A G. Vozzi %A Tirella, A %A A. Ahluwalia %K Bioengineering %B Methods in Molecular Biology %V 868 %P 57–69 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Realization and characterization of bilayer scaffold with molecularly imprinted hydrogel layer, %A Morelli, L %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 65 - 66 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2012 Atti %D 2012 %T Rheological characterization of vitreous humoranalogous %A Fogli, G %A G. Orsi %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2012 Atti %C Roma, Italy %P 207 - 208 %G eng %0 Journal Article %J ADVANCED MATERIALS %D 2011 %T Cooperation of Biological and Mechanical Signals in Cardiac Progenitor Cell Differentiation %A S. Pagliari %A A.C. Vilela-Silva %A G. Forte %A F. Pagliari %A C. Mandoli %A G. Vozzi %A S. Pietronave %A M. Prat %A S. Licoccia %A A. Ahluwalia %A E. Traversa %A M. Minieri %A P. Di Nardo %K Bioengineering %B ADVANCED MATERIALS %G eng %0 Journal Article %J Biotechnol Bioeng %D 2011 %T A flexible bioreactor system for constructing in vitro tissue and organ models. %A Vozzi, F. %A D. Mazzei %A Vinci, B. %A G. Vozzi %A Sbrana, T. %A Ricotti, L. %A Forgione, N. %A A. Ahluwalia %K Bioengineering %X

To develop in vitro models of cells, tissues and organs we have designed and realized a series of cell culture chambers. Each chamber is purpose designed to simulate a particular feature of the in vivo environment. The bioreactor system is user friendly, and the chambers are easy to produce, sterilize and assemble. In addition they can be connected together to simulate inter-organ or tissue cross-talk. Here we discuss the design philosophy of the bioreactor system and then describe its construction. Preliminary results of validation tests obtained with hepatocytes and endothelial cells are also reported. The results show that endothelial cells are extremely sensitive to small levels of shear stress and that the presence of heterotypic signals from endothelial cells enhances the endogenous metabolic function of hepatocytes.

%B Biotechnol Bioeng %V 108 %P 2129–2140 %8 Sep %G eng %R http://dx.doi.org/10.1002/bit.23164 %0 Journal Article %J COMPUTER METHODS IN BIOMECHANICS AND BIOMEDICAL ENGINEERING %D 2011 %T HEMETBeta: improvement of hepatocyte metabolism mathematical model %A G. Orsi %A C. De Maria %A M. A. Guzzardi %A Vozzi, F. %A G. Vozzi %K Bioengineering %B COMPUTER METHODS IN BIOMECHANICS AND BIOMEDICAL ENGINEERING %G eng %0 Patent %D 2011 %T High throughput sensorized bioreactor for applying hydrodynamic pressure and shear stress stiumli on cell cutlures %A A. Ahluwalia %A C. De Maria %A D. Mazzei %A G. Vozzi %K Bioengineering %8 10/2011 %G eng %0 Conference Paper %B Euromat-FEMS %D 2011 %T How dimension a substrate for the realisation of controlled micro- and bio-inspired environment %A Tirella, A %A A. Ahluwalia %A C. De Maria %A Vozzi, F. %A G. Vozzi %A Sandri, T %A Sassano, D %A Cognolato, L %K Bioengineering %X

European Congress and Exhibition on Advanced Materials and Processes - Oral

%B Euromat-FEMS %C Montpellier, France %0 Journal Article %J IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING %D 2011 %T LTI Models for 3-Iodothyronamine Time Dynamics: A Multiscale View. %A G. Orsi %A Frascarelli, S %A R. Zucca %A G. Vozzi %K Bioengineering %B IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING %V 58 %P 3153 - 3157 %G eng %0 Journal Article %J VETERINARY RESEARCH %D 2011 %T Melt Extruded PU Hollow Fibers for Nerve Regeneration: In vivo Study %A G. Vozzi %A F. Dini %A S. Burchielli %A C. Salvadori %A C. De Maria %A V. Chiono %A G. Barsotti %A G. Ciardelli %A F. Carlucci %A R. Roni %K Bioengineering %B VETERINARY RESEARCH %G eng %0 Conference Paper %B Euromat-FEMS %D 2011 %T Modelling and realisation of smart graded hydrogel scaffolds %A Tirella, A %A G. Mattei %A F. Montemurro %A G. Vozzi %A A. Ahluwalia %K Bioengineering %X

European Congress and Exhibition on Advanced Materials and Processes - Poster F12-P-2-08

%B Euromat-FEMS %C Montpellier, France %0 Journal Article %J Tissue Eng Part C Methods %D 2011 %T PAM2 (Piston Assisted Microsyringe): A New Rapid Prototyping Technique for Biofabrication of Cell Incorporated Scaffolds %A Tirella, A %A Vozzi, F. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B Tissue Eng Part C Methods %V 17 %G eng %R 10.1089/ten.tec.2010.0195 %0 Journal Article %J MACROMOLECULAR BIOSCIENCE %D 2011 %T Poly(ester urethane) Guides for Peripheral Nerve Regeneration %A V. Chiono %A Sartori, S %A Rechichi, A %A Tonda-Turo, C %A G. Vozzi %A Vozzi, F. %A D'Acunto, M %A C. Salvadori %A F. Dini %A G. Barsotti %A F. Carlucci %A S. Burchielli %A Nicolino, S %A Audisio, C %A Perroteau, I %A P. Giusti %A G. Ciardelli %K Bioengineering %B MACROMOLECULAR BIOSCIENCE %G eng %0 Journal Article %J BIOSENSORS & BIOELECTRONICS %D 2011 %T Sensing scaffolds to monitor cellular activity using impedance measurements %A Whulanza, Y %A Ucciferri, N %A C. Domenici %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B BIOSENSORS & BIOELECTRONICS %G eng %0 Journal Article %J Journal of bioscience and bioengineering %D 2011 %T Substrate stiffness influences high resolution printing of living cells with an ink-jet system %A Tirella, A %A Vozzi, F. %A C. De Maria %A G. Vozzi %A Sandri, T %A Sassano, D %A Cognolato, L %A A. Ahluwalia %K Bioengineering %X

The adaptation of inkjet printing technology for the realisation of controlled micro- and nano-scaled biological structures is of great potential in tissue and biomaterial engineering. In this paper we present the Olivetti BioJet system and its applications in tissue engineering and cell printing. BioJet, which employs a thermal inkjet cartridge, was used to print biomolecules and living cells. It is well known that high stresses and forces are developed during the inkjet printing process. When printing living particles (i.e., cell suspensions) the mechanical loading profile can dramatically damage the processed cells. Therefore computational models were developed to predict the velocity profile and the mechanical load acting on a droplet during the printing process. The model was used to investigate the role of the stiffness of the deposition substrate during droplet impact and compared with experimental investigations on cell viability after printing on different materials. The computational model and the experimental results confirm that impact forces are highly dependent on the deposition substrate and that soft and viscous surfaces can reduce the forces acting on the droplet, preventing cell damage. These results have high relevance for cell bioprinting; substrates should be designed to have a good compromise between substrate stiffness to conserve spatial patterning without droplet coalescence but soft enough to absorb the kinetic energy of droplets in order to maintain cell viability.

%B Journal of bioscience and bioengineering %V 112 %P 79–85 %G eng %U http://www.ncbi.nlm.nih.gov/pubmed/21497548 %R 10.1016/j.jbiosc.2011.03.019 %0 Journal Article %J MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS %D 2011 %T Tuning polycaprolactone-carbon nanotube composites for bone tissue engineering scaffolds %A Mattioli-Belmonte, M. %A G. Vozzi %A Whulanza, Y %A Seggiani, M %A Fantauzzi, V %A Orsini, G %A A. Ahluwalia %K Bioengineering %B MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS %G eng %0 Journal Article %J EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY %D 2010 %T Automated software for analysis of ciliary beat frequency and metachronal wave orientation in Primary Ciliary Dyskinesia %A G. MANTOVANI %A M. PIFFERI %A G. Vozzi %K Bioengineering %B EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY %G eng %0 Conference Paper %B Congress of the European Society of Biomechanics, ESB 2010 %D 2010 %T Engineered Skeletal Muscle using Pam Scaffold %A D. Cei %A E. Loro %A A. Malena %A C. De Maria %A L. Vergani %A G. Vozzi %K Bioengineering %B Congress of the European Society of Biomechanics, ESB 2010 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T Enzymatic cross-linked hydrogel: role of Lysyl oxidase as an initiator of fibroblast inflammatory response %A Tirella, A %A G. Vozzi %A N. Tirelli %A A. Ahluwalia %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %D 2010 %T Enzymatically crosslinked porous composite matrices for bone tissue regeneration %A G. Ciardelli %A GENTILE, P %A V. Chiono %A Mattioli-Belmonte, M. %A G. Vozzi %A Barbani, N %A P. Giusti %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %G eng %0 Journal Article %J Toxicology in vitro %D 2010 %T Finite element modelling and design of a concentration gradient generating bioreactor: Application to biological pattern formation and toxicology %A G. Vozzi %A D. Mazzei %A Tirella, A %A Vozzi, F. %A A. Ahluwalia %K Bioengineering %X

This paper describes the use of a microfluidic gradient maker for the toxicological analysis of some conventional biomolecules such as hydrogen peroxide and a local anaesthetic, lidocaine on different cell cultures, human endothelial cells and myoblasts, respectively. The microfluidic device was designed and simulated using COMSOL Multiphysics(R) and the concentration gradient in the microfluidic network was analysed through a fluid-dynamic and mass-transport study. Subsequently the device was fabricated with soft lithography, casting PDMS in a master to obtain channels about 250mum deep. Hydrogen peroxide was tested on human endothelial cells, while lidocaine was tested on C2C12 myoblasts and an analysis was performed using propidium iodide staining followed by an imaging processing routine to obtain quantitative dose-response profiles in the gradient maker. The results show that the Gradient Maker (GM) bioreactor is a more sensitive method for detection of cell toxicity, and compared with testing of drug toxicity using microwells with individual cell cultures, allows one shot testing with a single cell culture exposed to a large number of concentrations. Moreover, the Gradient Maker was also modelled in order to realise biological pattern formation using two morphogenes acting as activator and inhibitor with varying diffusion rates.

%B Toxicology in vitro %V 24 %P 1828–1837 %G eng %U http://www.ncbi.nlm.nih.gov/pubmed/20580814 %R 10.1016/j.tiv.2010.05.010 %0 Conference Paper %B NIP Conference %D 2010 %T Hydrogel Bioactive Scaffold Fabricated with PAM2 System: Realization of Complex Shaped Scaffold with a Homogeneous Dispersion of HepG2 cells %A Tirella, A %A Vozzi, F. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B NIP Conference %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T In silico model of hepatic metabolism of Diclofenac %A A. Moschetti %A F. Montemurro %A C. De Maria %A Vozzi, F. %A A. Ahluwalia %A G. Vozzi %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Journal Article %J Biotechnol J %D 2010 %T In vitro liver model using microfabricated scaffolds in a modular bioreactor. %A Vinci, B. %A Cavallone, D. %A G. Vozzi %A D. Mazzei %A C. Domenici %A Brunetto, M. %A A. Ahluwalia %K Bioengineering %X

Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cell interactions are mediated by the architecture of the substrate, while the endogenous biochemical functions are regulated by a sustainable supply of nutrients and interstitial-like flow. Thus, a combination of 3-D scaffolds and dynamic flow conditions are both important for the development of a hepatic tissue model for applications in drug testing and regenerative medicine.

%B Biotechnol J %V 5 %P 232–241 %8 Feb %G eng %R http://dx.doi.org/10.1002/biot.200900074 %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T Mechanical characterization of polymer cartilage scaffold at different pH %A S. Romiti %A G. Vozzi %A C. De Maria %A F. Montemurro %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Journal Article %J SENSORS AND ACTUATORS. B, CHEMICAL %D 2010 %T Molecularly imprinted nanospheres for applications in a novel model biosensor-scaffold, %A MORELLI, I %A V. Chiono %A G. Vozzi %A G. Ciardelli %A SILVESTRI, D %A P. Giusti %K Bioengineering %B SENSORS AND ACTUATORS. B, CHEMICAL %G eng %0 Journal Article %J BIOMEDICAL ENGINEERING. APPLICATIONS, BASIS, COMMUNICATIONS %D 2010 %T NEW BIOARTIFICIAL SYSTEMS AND BIODEGRADABLE SYNTHETIC POLYMERS FOR CARDIAC TISSUE ENGINEERING: A PRELIMINARY SCREENING %A Rosellini, E %A Cristallini, C %A Barbani, N %A G. Vozzi %A D'Acunto, M %A G. Ciardelli %A P. Giusti %K Bioengineering %B BIOMEDICAL ENGINEERING. APPLICATIONS, BASIS, COMMUNICATIONS %G eng %0 Journal Article %J JOURNAL OF OSTEOLOGY AND BIOMATERIALS %D 2010 %T Novel injectable hydrogel scaffold for cartilage repair based on natural polymers %A G. Mattei %A F. Montemurro %A Mattioli-Belmonte, M. %A G. Vozzi %K Bioengineering %B JOURNAL OF OSTEOLOGY AND BIOMATERIALS %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T Pam scaffold for heart tissue engineering %A M. Nardi %A C. De Maria %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T Pam Scaffold For Tissue Engineering Of Skeletal Muscle %A D. Cei %A E. Loro %A A. Malena %A L. Vergani %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T PAM2 microfabricated three-dimensional bioactive hydrogel systems: realisation of a hepatic-like structure %A Tirella, A %A Vozzi, F. %A Vinci, B. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Conference Paper %B 2010 International Conference on Biofabrication, 4-6 October 2010, Philadelphia, USA %D 2010 %T PAM2 System: a Modular Approach for the Realisation of Complex Shaped Scaffold Able to Reproduce the Main Features of a Specific Micro-environment, %A A. Ahluwalia %A Tirella, A %A G. Vozzi %K Bioengineering %B 2010 International Conference on Biofabrication, 4-6 October 2010, Philadelphia, USA %G eng %0 Book Section %B Computer-Aided Tissue Engineering %D 2010 %T Rapid Prototyping Composite and Complex Scaffolds with PAM2 %A G. Vozzi %A Tirella, A %A A. Ahluwalia %K Bioengineering %B Computer-Aided Tissue Engineering %V 868 %P 57-69 %G eng %& Rapid Prototyping Composite and Complex Scaffolds with PAM2 %0 Conference Paper %B Congress of the European Society of Biomechanics, ESB 2010 %D 2010 %T Resident cardiac stem cells on PAM scaffold for Heart tissue engineering application %A M. Nardi %A C. De Maria %A G. Forte %A P. Di Nardo %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Congress of the European Society of Biomechanics, ESB 2010 %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T Sensorised "Smart" Scaffold to Monitor Cell Processes Based on Impedance Characteristics %A Whulanza, Y %A Ucciferri, N %A G. Vozzi %A A. Ahluwalia %A C. Domenici %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Conference Paper %B NIP Conference %D 2010 %T Site Specific Nano-Tuning of Scaffolds Using Inkjet Printing %A Tirella, A %A F. Montemurro %A Vinci, B. %A Vozzi, F. %A G. Vozzi %A Sassano, D %A Sandri, T %A Cognolato, L %A A. Ahluwalia %K Bioengineering %B NIP Conference %C Austin, Texas %G eng %0 Journal Article %J BIOTECHNOLOGY AND BIOENGINEERING %D 2010 %T SOFT-MI: a novel microfabrication technique integrating Soft-lithography and Molecular imprinting for tissue engineering applications %A G. Vozzi %A MORELLI, I %A Vozzi, F. %A ANDREONI, C %A SALSEDO, E %A MORACHIOLI, A %A P. Giusti %A G. Ciardelli %K Bioengineering %B BIOTECHNOLOGY AND BIOENGINEERING %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2010 %D 2010 %T Soft-MI scaffold for tissue engineering applications %A MORACHIOLI, A %A G. Vozzi %A F. Montemurro %K Bioengineering %B Congresso Nazionale di Bioingegneria 2010 %G eng %0 Journal Article %J INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS %D 2010 %T Three-dimensional microfabricated scaffolds with cardiac extracellular matrix-like architecture %A Rossellini, E %A G. Vozzi %A Barbani, N %A P. Giusti %A Cristallini, C %K Bioengineering %B INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS %G eng %0 Journal Article %J ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY %D 2009 %T Development of a RGDS-peptide modified polyurethane for tissue regeneration %A Rechichi, A %A Sartori, S %A CAPORALE, A %A G. Ciardelli %A G. Vozzi %A MAZZUCCO, L %A PEGGION, E %A P. Giusti %K Bioengineering %B ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY %V 611 %P 249–250 %G eng %0 Conference Paper %B 2009 Ninth International Conference on Intelligent Systems Design and Applications %D 2009 %T ENMET: Endothelial Cell Metabolism Mathematical Model, %A G. Orsi %A C. De Maria %A Vozzi, F. %A M. A. Guzzardi %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B 2009 Ninth International Conference on Intelligent Systems Design and Applications %P 654 - 659 %G eng %0 Journal Article %J BIOMEDICAL MICRODEVICES %D 2009 %T Melt-extruded guides for peripheral nerve regeneration. Part I: Poly(epsilon-caprolactone) %A V. Chiono %A G. Vozzi %A Vozzi, F. %A C. Salvadori %A F. Dini %A F. Carlucci %A ARISPICI, M %A S. Burchielli %A DI SCIPIO, F %A GEUNA, S %A FORNARO, M %A TOS, P %A Nicolino, S %A Audisio, C %A Perroteau, I %A CHIARAVALLOTI, A %A C. Domenici %A P. Giusti %A G. Ciardelli %K Bioengineering %B BIOMEDICAL MICRODEVICES %P 1037-1050 %G eng %0 Journal Article %J COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE %D 2009 %T A new library of HEMET model: Insulin effects on hepatic metabolism %A CUTRONE, A %A C. De Maria %A Vinci, B. %A Vozzi, F. %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE %V 94 %P 181–189 %G eng %N 2 %R 10.1016/j.cmpb.2008 %0 Journal Article %J Biofabrication %D 2009 %T A phase diagram for microfabrication of geometrically controlled hydrogel scaffolds %A Tirella, A %A Orsini, A %A G. Vozzi %A A. Ahluwalia %K Bioengineering %X

Hydrogels are considered as excellent candidates for tissue substitutes by virtue of their high water content and biphasic nature. However, the fact that they are soft, wet and floppy renders them difficult to process and use as custom-designed scaffolds. To address this problem alginate hydrogels were modeled and characterized by measuring stress-strain and creep behavior as well as viscosity as a function of sodium alginate concentration, cross-linking time and calcium ion concentration. The gels were then microfabricated into scaffolds using the pressure-assisted microsyringe. The mechanical and viscous characteristics were used to generate a processing window in the form of a phase diagram which describes the fidelity of the scaffolds as a function of the material and machine parameters. The approach can be applied to a variety of microfabrication methods and biomaterials in order to design well-controlled custom scaffolds.

%B Biofabrication %V 1 %P 045002 %G eng %U http://www.ncbi.nlm.nih.gov/pubmed/20811111 %R 10.1088/1758-5082/1/4/045002 %0 Conference Paper %B 3B International Conference in Bioprinting and Biofabrication %D 2009 %T Pneumatic module of PAM2 microfabrication system: realization of bio-inspired complex scaffolds %A C. De Maria %A Tirella, A %A A. Ahluwalia %A G. Vozzi %K Bioengineering %X

poster

%B 3B International Conference in Bioprinting and Biofabrication %C Bordeaux, France %0 Journal Article %J MATERIALS SCIENCE AND ENGINEERING. C %D 2009 %T Polyurethane unimorph bender microfabricated with Pressure Assisted Microsyringe (PAM) for biomedical applications %A Tartarisco, G. %A G. GALLONE %A F. Carpi %A G. Vozzi %K Bioengineering %B MATERIALS SCIENCE AND ENGINEERING. C %V 29 %P 1835–1841 %G eng %N 6 %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %D 2009 %T Preparation and characterization of alginate/gelatin blend films for cardiac tissue engineering %A Rosellini, E %A Cristallini, C %A Barbani, N %A G. Vozzi %A P. Giusti %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %V 91 %P 447-453 %G eng %N 2 %R 10.1002/jbm.a.32216 %0 Journal Article %J THORAX %D 2009 %T Simplified cell culture method for the diagnosis of atypical Primary Ciliary %A M. PIFFERI %A F. Montemurro %A CANGIOTTI, A M %A RAGAZZO, V %A DI CICCO, M %A Vinci, B. %A G. Vozzi %A MACCHIA, P %A BONER, A L %K Bioengineering %B THORAX %V 64 %P 1077-81 %G eng %R 10.1136/thx.2008 %0 Conference Paper %B Discinesie ciliarie e malattie respiratorie croniche del bambino: dalla diagnosi differenziale alla qualità  della vita %D 2008 %T Analisi del battito ciliare nella discinesia ciliare tramite algoritmi di motion detection %A G. MANTOVANI %A M. A. Guzzardi %A RAGAZZO, V %A M. PIFFERI %A G. Vozzi %K Bioengineering %B Discinesie ciliarie e malattie respiratorie croniche del bambino: dalla diagnosi differenziale alla qualità  della vita %C 14-15 March 2008, Pisa, Italy %P – %G eng %0 Book Section %D 2008 %T Biomaterials fabrication and processing handbook %A G. Vozzi %A A. Ahluwalia %K Bioengineering %P – %G eng %& Rapid Prototyping Methods for Tissue Engineering Applications %0 Conference Paper %B International Conference on Digital Printing Technologies Conference Proceedings %D 2008 %T Biomimicry of PAM Microfabricated Hydrogel Scaffolds %A Tirella, A %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B International Conference on Digital Printing Technologies Conference Proceedings %P – %G eng %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %D 2008 %T Chitosan/gelatin blends for biomedical applications %A PULIERI, E %A V. Chiono %A G. Ciardelli %A G. Vozzi %A A. Ahluwalia %A C. Domenici %A Vozzi, F. %A P. Giusti %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %V 86 %P 311–322 %G eng %0 Journal Article %J INTERNAL MEDICINE %D 2008 %T Comparative study of porous and engineered biomaterials %A Mattioli-Belmonte, M. %A K KYRIAKIDOU %A LUCARINI, G %A ZIZZI, A %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B INTERNAL MEDICINE %V 15 %P 4–8 %G eng %0 Conference Paper %D 2008 %T Composite polyurethane and carbon black bimorph bender microfabricated with pressure assisted microsyringe (PAM) for biomedical applications %A Tartarisco, G. %A G. GALLONE %A F. Carpi %A G. Vozzi %K Bioengineering %G eng %0 Journal Article %J BIOMEDICAL MICRODEVICES %D 2008 %T Controlled in vitro growth of cell microtubes: towards the realisation of artificial microvessels %A Migliore, A. %A Vozzi, F. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B BIOMEDICAL MICRODEVICES %G eng %0 Journal Article %J STEM CELLS %D 2008 %T Criticality of the Biological and Physical Stimuli Array Inducing Resident Cardiac Stem Cell Determination %A G. Forte %A CAROTENUTO, F %A F. Pagliari %A S. Pagliari %A COSSA, P %A FIACCAVENTO, R %A A. Ahluwalia %A G. Vozzi %A Vinci, B. %A SERAFINO, A %A RINALDI, A %A E. Traversa %A CAROSELLA, L %A M. Minieri %A P. Di Nardo %K Bioengineering %B STEM CELLS %V 26 %P 2093–2103 %G eng %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %D 2008 %T Degradable block polyurethanes from nontoxicbuilding blocks as scaffold materials to support cell growth and proliferation %A Rechichi, A %A G. Ciardelli %A D'Acunto, M %A G. Vozzi %A P. Giusti %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %P – %G eng %0 Journal Article %J MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS %D 2008 %T Electroactive Carbon Nanotube Actuators: Soft-Lithographic Fabrication and Electro-chemical Modelling %A A. Mazzoldi %A M. Tesconi %A A. Tognetti %A W. Rocchia %A G. Vozzi %A G. Pioggia %A A. Ahluwalia %A D. De Rossi %K Bioengineering %B MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS %P – %G eng %0 Journal Article %J ENGINEERING IN LIFE SCIENCES %D 2008 %T Enzymatically-modified melt-extruded guides for peripheral nerve repair %A V. Chiono %A G. Ciardelli %A G. Vozzi %A CORTEZ, J %A Barbani, N %A GENTILE, P %A P. Giusti %K Bioengineering %B ENGINEERING IN LIFE SCIENCES %G eng %0 Journal Article %J JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE %D 2008 %T Genipin-crosslinked chitosan/gelatin blends for biomedical applications %A V. Chiono %A PULIERI, E %A G. Vozzi %A G. Ciardelli %A A. Ahluwalia %A P. Giusti %K Bioengineering %B JOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE %G eng %0 Journal Article %J COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE %D 2008 %T HEMET: mathematical model of biochemical pathways for simulation and prediction of HEpatocyte METabolism %A C. De Maria %A GRASSINI, D %A Vozzi, F. %A Vinci, B. %A A. Landi %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE %V 92 %P 121–134 %G eng %0 Journal Article %J IEEE T Ind Electron %D 2008 %T A High-Throughput Bioreactor System for Simulating Physiological Environments %A D. Mazzei %A Vozzi, F. %A Cisternino, A. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B IEEE T Ind Electron %V 55 %P 3273–3280 %G eng %R 10.1109/TIE.2008.928122 %0 Journal Article %J INTERNAL MEDICINE %D 2008 %T In vitro engineering of the liver using multiple axis stimuli %A Vinci, B. %A G. Vozzi %A AVOGARO, A %A A. Ahluwalia %K Bioengineering %B INTERNAL MEDICINE %V 15 %P 13–15 %G eng %0 Conference Paper %B Congresso Nazionale Biomateriali 2008 %D 2008 %T Microfabbricazione di scaffold in alginato con proprietà  meccaniche modulabili %A Orsini, A %A Tirella, A %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Congresso Nazionale Biomateriali 2008 %P – %G eng %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2008 %D 2008 %T Microfabrication of Hydrogels scaffolds including cells and mechanical characterization %A Tirella, A %A G. Vozzi %K Bioengineering %B Congresso Nazionale di Bioingegneria 2008 %C Pisa, Italy %P 367-368 %G eng %0 Conference Paper %B TERMIS-EU %D 2008 %T Microfluidic Gradient Maker for Pattern Generation %A Tirella, A %A D. Mazzei %A G. Vozzi %A A. Ahluwalia %K Bioengineering %X

poster

%B TERMIS-EU %C Porto, Portugal %P 879 %0 Conference Paper %B Congresso Nazionale di Bioingegneria 2008 %D 2008 %T Nanotechnology in Tissue Engineering %A G. Ciardelli %A V. Chiono %A Rechici, A %A Sartori, S %A G. Vozzi %A Bignardi, C %A Georgiev, G %A P. Giusti %K Bioengineering %B Congresso Nazionale di Bioingegneria 2008 %P – %G eng %0 Conference Paper %D 2008 %T Nerve guide for peripheral nerve re generation %A Tonda-Turo, C %A V. Chiono %A GEUNA, S %A Perroteau, I %A G. Vozzi %A G. Ciardelli %K Bioengineering %G eng %0 Journal Article %J Biomedicine and Pharmacotherapy %D 2008 %T Nerve regeneration using novel biomaterial supports %A G. Vozzi %A V. Chiono %A Vozzi, F. %A C. Salvadori %A F. Dini %A F. Carlucci %A ARISPICI, M %A S. Burchielli %A G. Ciardelli %A P. Giusti %K Bioengineering %B Biomedicine and Pharmacotherapy %P – %G eng %0 Conference Paper %B Biomedicine & Pharmacotherapy %D 2008 %T Nerve regeneration using novel biomaterial supports %A G. Vozzi %A V. Chiono %A Vozzi, F. %A C. Salvadori %A F. Dini %A F. Carlucci %A ARISPICI, M %A S. Burchielli %A G. Ciardelli %A P. Giusti %K Bioengineering %B Biomedicine & Pharmacotherapy %P – %G eng %0 Journal Article %J MULTIDISCIPLINE MODELING IN MATERIALS AND STRUCTURES %D 2008 %T A New Bio-Inspired Robot Based on Senseless Motion: Theoretical study and Preliminary Technological Results %A COTRONEO, A %A G. Vozzi %A GEROVASI, L %A D. De Rossi %K Bioengineering %B MULTIDISCIPLINE MODELING IN MATERIALS AND STRUCTURES %G eng %0 Journal Article %J TISSUE ENGINEERING, PART A %D 2008 %T Optimization of PAM Scaffolds for Neural Tissue Engineering: Preliminary Study on an SH-SY5Y Cell Line %A KULLENBERG, J %A ROSATINI, F %A G. Vozzi %A BIANCHI, F %A A. Ahluwalia %A C. Domenici %K Bioengineering %B TISSUE ENGINEERING, PART A %G eng %0 Conference Paper %B National Conference of Bioengineering %D 2008 %T PAM Composite Scaffold of PCL and Carbon NanoTubes for Bone Tissue Regeneration %A Mattioli-Belmonte, M. %A Fantauzzi, V %A K KYRIAKIDOU %A Tirella, A %A A. Ahluwalia %A G. Vozzi %K Bioengineering %X

poster

%B National Conference of Bioengineering %C Pisa, Italy %P 369 %0 Journal Article %J MACROMOLECULAR BIOSCIENCE %D 2008 %T PAM-microfabricated polyurethane scaffolds: in vivo and in vitro preliminary studies %A G. Vozzi %A Rechichi, A %A F. Dini %A C. Salvadori %A Vozzi, F. %A S. Burchielli %A F. Carlucci %A ARISPICI, M %A G. Ciardelli %A P. Giusti %A A. Ahluwalia %K Bioengineering %B MACROMOLECULAR BIOSCIENCE %G eng %0 Journal Article %J IEEE INDUSTRIAL ELECTRONICS MAGAZINE %D 2008 %T A patented drop-free trocar for ophthalmic applications: design and realization [from mind to market] %A Guerrini, P. %A G. Vozzi %A A. Ahluwalia %A Palla, M. %K Bioengineering %B IEEE INDUSTRIAL ELECTRONICS MAGAZINE %V 2 %P 4–8 %G eng %0 Journal Article %J IEEE INDUSTRIAL ELECTRONICS MAGAZINE %D 2008 %T A patented drop-free trocar for ophthalmic applications: design and realization [from mind to market] %A Guerrini, P. %A G. Vozzi %A A. Ahluwalia %A Palla, M. %A G. Vozzi %B IEEE INDUSTRIAL ELECTRONICS MAGAZINE %V 2 %P 4–8 %G eng %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %D 2008 %T Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(epsilon-caprolactone) blends for tissue engineering applications in the form of hollow fibers %A V. Chiono %A G. Ciardelli %A G. Vozzi %A SOTGIU, MG %A Vinci, B. %A C. Domenici %A P. Giusti %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %P – %G eng %0 Conference Paper %D 2008 %T Quantitative assesment of nerve reneration by electromyographical and electroneurographical signals %A C. De Maria %A D. S. POGGI %A S. Burchielli %A G. Vozzi %G eng %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %D 2008 %T Rapid-prototyped and salt-leached PLGA scaffolds condition cell morpho-functional behaviour %A Mattioli-Belmonte, M. %A G. Vozzi %A K KYRIAKIDOU %A PULIERI, E %A LUCARINI, G %A Vinci, B. %A PUGNALONI, A %A BIAGINI, G %A A. Ahluwalia %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A %P – %G eng %0 Conference Paper %B Congresso Nazionale Biomateriali 2008 %D 2008 %T Realizzazione di strutture micro fabbricate tramite tecnica di micro Laser Sintering per applicazione alla Tissue Engineering %A G. Criscenti %A Tirella, A %A A. Ahluwalia %A P. Giusti %A G. Vozzi %K Bioengineering %B Congresso Nazionale Biomateriali 2008 %P – %G eng %0 Journal Article %J REACTIVE & FUNCTIONAL POLYMERS %D 2008 %T Surface modification of a synthetic polyurethane by plasma glow discharge: preparation and characterisation of bioactive monolayers %A Sartori, S %A Rechichi, A %A G. Vozzi %A D'Acunto, M %A HEINE, E %A P. Giusti %A G. Ciardelli %K Bioengineering %B REACTIVE & FUNCTIONAL POLYMERS %V 68 %P 809–821 %G eng %0 Conference Paper %B Congresso Nazionale Biomateriali 2008 %D 2008 %T Sviluppo e caratterizzazione meccanica di scaffold a base di collagene per la rigenerazione della cartilagine %A G. Mattei %A F. Montemurro %A A. Ahluwalia %A G. Vozzi %K Bioengineering %B Congresso Nazionale Biomateriali 2008 %P – %G eng %0 Book Section %D 2008 %T Tissue Engineering: Roles, Materials and Applications %A V. Chiono %A G. Vozzi %A G. Ciardelli %K Bioengineering %P – %G eng %& Gellan/Gelatin-Based Biomaterials for Tissue Engineering %0 Journal Article %J EUROPEAN JOURNAL OF NEUROSCIENCE %D 2007 %T Acute retinal ganglion cell injury caused by intraocular pressure spikes is mediated by endogenous extracellular ATP %A RESTA, V. %A NOVELLI, E. %A G. Vozzi %A SCARPA, C. %A CALEO, M. %A A. Ahluwalia %A SOLINI, A. %A SANTINI, E. %A PARISI, V. %A DI VIRGILIO, F. %A GALLI-RESTA, L. %K Bioengineering %B EUROPEAN JOURNAL OF NEUROSCIENCE %V 25 %P 2741–2754 %G eng %0 Conference Paper %B Biotechnical functionalisation of renewable polymeric materials %D 2007 %T Bioactive polyurethanes trough new functionalisation stretegies %A G. Ciardelli %A Sartori, S %A Rechichi, A %A CAPORALE, A %A S. BRONCO %A G. Vozzi %A P. Giusti %K Bioengineering %B Biotechnical functionalisation of renewable polymeric materials %8 16-17 April %G eng %0 Conference Paper %B European Polymer Congress 2007 %D 2007 %T Biomimetic materials for tissue engineering through enzyme mediated protein modification %A G. Ciardelli %A GENTILE, P %A Mandrile, R. %A V. Chiono %A Barbani, N %A Mattioli-Belmonte, M. %A G. Vozzi %A P. Giusti %K Bioengineering %B European Polymer Congress 2007 %C Portoroz, Slovenia %8 2-6 July %G eng %0 Conference Paper %B Termis Eu-Meeting London (UK) 4-7 September 2007 Tissue Engineering 2007, 13 (7): 1647 %D 2007 %T Cardiac and Mesenchymal Stem Cell Growth and Selective Differentiation on Three Dimensional Bioerodable Scaffolds %A G. Forte %A CAROTENUTO, F %A G. Vozzi %A COSSA, P %A FIACCAVENTO, R %A M. Minieri %A F. Pagliari %A S. Pagliari %A Romano, R. %A A. Ahluwalia %A E. Traversa %A P. Di Nardo %K Bioengineering %B Termis Eu-Meeting London (UK) 4-7 September 2007 Tissue Engineering 2007, 13 (7): 1647 %P – %G eng %0 Conference Paper %B Proc. IEEE Int. Symp. Industrial Electronics ISIE 2007 %D 2007 %T Design and realisation of drop-free trocar for ophthalmic applications %A Guerrini, P. %A G. Vozzi %A A. Ahluwalia %A D. Mazzei %A Palla, M. %A Rizzo, S. %K Bioengineering %B Proc. IEEE Int. Symp. Industrial Electronics ISIE 2007 %P 2810–2814 %G eng %R 10.1109/ISIE.2007.4375056 %0 Conference Paper %B Proc. IEEE Int. Symp. Industrial Electronics ISIE 2007 %D 2007 %T Development of a high-throughput bioreactor system for biomedical applications %A D. Mazzei %A G. Vozzi %A A. Ahluwalia %A Cisternino, A. %K Bioengineering %B Proc. IEEE Int. Symp. Industrial Electronics ISIE 2007 %P 2788–2792 %G eng %R 10.1109/ISIE.2007.4375051 %0 Conference Paper %B Termis Eu-Meeting - Tissue Engineering 2007 %D 2007 %T Hydroxyapatite/collagen composite matrices for bone reconstruction %A G. Ciardelli %A GENTILE, P %A Mandrile, R. %A V. Chiono %A Barbani, N %A Mattioli-Belmonte, M. %A G. Vozzi %A P. Giusti %K Bioengineering %B Termis Eu-Meeting - Tissue Engineering 2007 %C London (UK) %P 13 (7): 1708 %G eng %0 Journal Article %J RIVISTA DI CHIRURGIA DELLA MANO %D 2007 %T La rigenerazione nervosa negli innesti a polarità  invertita: dati a confronto tra applicazione sull'uomo e analisi sperimentale su modello animale %A D. S. POGGI %A Massarella, M. %A Cerulli, G. %A Caraffa, A. %A S. Burchielli %A Modenato, M. %A Cantile, C. %A G. Vozzi %A C. De Maria %A Lisanti, M. %A Bonicoli, E. %A Cantini, G. %K Bioengineering %B RIVISTA DI CHIRURGIA DELLA MANO %P – %G eng %0 Journal Article %J JOURNAL OF MATERIALS CHEMISTRY %D 2007 %T Microfabrication for tissue engineering: rethinking the cells-on-a scaffold approach %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B JOURNAL OF MATERIALS CHEMISTRY %P – %G eng %0 Journal Article %J JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS %D 2007 %T Microfabrication of fractal polymeric structures for capillary morphogenesis: applications in therapeutic angiogenesis and in the engineering of vascularized tissue %A BIANCHI, F %A ROSI, M. %A G. Vozzi %A EMANUELI, C. %A MADEDDU, P. %A A. Ahluwalia %K Bioengineering %B JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART B, APPLIED BIOMATERIALS %V 81 %P 462–468 %G eng %0 Conference Paper %B European Polymer Congress 2007 %D 2007 %T Molecularly Imprinted Polymers with Recognition Properties Toward Fibronectin H-Gly-Arg-Gly-Asp-Ser-OH Sequence %A Rechichi, A %A Rosellini, E %A G. Ciardelli %A Barbani, N %A G. Vozzi %A P. Giusti %A Cristallini, C %K Bioengineering %B European Polymer Congress 2007 %C Portoroz, Slovenia %8 2-6 July %G eng %0 Journal Article %J JOURNAL OF CELLULAR AND MOLECULAR MEDICINE %D 2007 %T New biomedical devices with selective peptide recognition properties. Part 1: Characterization and cytotoxicity of molecularly imprinted polymers %A Rechichi, A %A Cristallini, C %A VITALE, U. %A G. Ciardelli %A Barbani, N %A G. Vozzi %A P. Giusti %K Bioengineering %B JOURNAL OF CELLULAR AND MOLECULAR MEDICINE %P – %G eng %0 Conference Paper %B Biosurf, 28-31 August 2007 %D 2007 %T New strategies in polymer functionalisation %A Sartori, S %A Rechichi, A %A G. Ciardelli %A CAPORALE, A %A G. Vozzi %A MAZZUCCO, L %K Bioengineering %B Biosurf, 28-31 August 2007 %C Zurich (Switzerland) %P European Cells and Materials Vol. 14. Suppl. 3 %G eng %0 Patent %D 2006 %T Sensore di flusso tattile e di parametri ambientali %A G. Vozzi %A Cisternino, A. %A Colombo, D. %A D. Mazzei %A Migliore, A. %K Bioengineering %G eng %0 Conference Paper %B Proceedings of: XIV Edizione del Congresso della Fisiologia Clinica-CNR %D 2005 %T Functional and Pharmacological testing of endothelial cells cultured in dynamic conditions %A Vozzi, F. %A Gentile, C. %A D. Mazzei %A G. Vozzi %A C. Domenici %A A. Ahluwalia %K Bioengineering %B Proceedings of: XIV Edizione del Congresso della Fisiologia Clinica-CNR %P 82-96 %G eng %0 Conference Paper %B Proceedings of: 4Th Annual Meeting of the European Tissue Engineering Society (ETES) %D 2005 %T Realisation of a concentration gradients cell chamber to study their effect of biomolecules on the cell activity %A D. Mazzei %A Vozzi, F. %A G. Vozzi %A A. Ahluwalia %K Bioengineering %B Proceedings of: 4Th Annual Meeting of the European Tissue Engineering Society (ETES) %G eng