TY - JOUR T1 - Realization of a poro-elastic ultrasound replica of pulmonary tissue JF - Biomatter Y1 - 2012 A1 - Spinelli, A A1 - Vinci, B. A1 - Tirella, A A1 - Matteucci, M A1 - Gargani, L A1 - A. Ahluwalia A1 - C. Domenici A1 - Picano, E A1 - Chiarelli, P KW - Bioengineering VL - 2 UR - http://www.landesbioscience.com/journals/biomatter/article/19835/ ER - TY - JOUR T1 - A flexible bioreactor system for constructing in vitro tissue and organ models. JF - Biotechnol Bioeng Y1 - 2011 A1 - Vozzi, F. A1 - D. Mazzei A1 - Vinci, B. A1 - G. Vozzi A1 - Sbrana, T. A1 - Ricotti, L. A1 - Forgione, N. A1 - A. Ahluwalia KW - Bioengineering AB -

To develop in vitro models of cells, tissues and organs we have designed and realized a series of cell culture chambers. Each chamber is purpose designed to simulate a particular feature of the in vivo environment. The bioreactor system is user friendly, and the chambers are easy to produce, sterilize and assemble. In addition they can be connected together to simulate inter-organ or tissue cross-talk. Here we discuss the design philosophy of the bioreactor system and then describe its construction. Preliminary results of validation tests obtained with hepatocytes and endothelial cells are also reported. The results show that endothelial cells are extremely sensitive to small levels of shear stress and that the presence of heterotypic signals from endothelial cells enhances the endogenous metabolic function of hepatocytes.

VL - 108 ER - TY - JOUR T1 - In vitro liver model using microfabricated scaffolds in a modular bioreactor. JF - Biotechnol J Y1 - 2010 A1 - Vinci, B. A1 - Cavallone, D. A1 - G. Vozzi A1 - D. Mazzei A1 - C. Domenici A1 - Brunetto, M. A1 - A. Ahluwalia KW - Bioengineering AB -

Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cell interactions are mediated by the architecture of the substrate, while the endogenous biochemical functions are regulated by a sustainable supply of nutrients and interstitial-like flow. Thus, a combination of 3-D scaffolds and dynamic flow conditions are both important for the development of a hepatic tissue model for applications in drug testing and regenerative medicine.

VL - 5 ER - TY - CONF T1 - PAM2 microfabricated three-dimensional bioactive hydrogel systems: realisation of a hepatic-like structure T2 - Congresso Nazionale di Bioingegneria 2010 Y1 - 2010 A1 - Tirella, A A1 - Vozzi, F. A1 - Vinci, B. A1 - G. Vozzi A1 - A. Ahluwalia KW - Bioengineering JF - Congresso Nazionale di Bioingegneria 2010 ER - TY - CONF T1 - Site Specific Nano-Tuning of Scaffolds Using Inkjet Printing T2 - NIP Conference Y1 - 2010 A1 - Tirella, A A1 - F. Montemurro A1 - Vinci, B. A1 - Vozzi, F. A1 - G. Vozzi A1 - Sassano, D A1 - Sandri, T A1 - Cognolato, L A1 - A. Ahluwalia KW - Bioengineering JF - NIP Conference CY - Austin, Texas ER - TY - JOUR T1 - A new library of HEMET model: Insulin effects on hepatic metabolism JF - COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE Y1 - 2009 A1 - CUTRONE, A A1 - C. De Maria A1 - Vinci, B. A1 - Vozzi, F. A1 - A. Ahluwalia A1 - G. Vozzi KW - Bioengineering VL - 94 IS - 2 ER - TY - JOUR T1 - Simplified cell culture method for the diagnosis of atypical Primary Ciliary JF - THORAX Y1 - 2009 A1 - M. PIFFERI A1 - F. Montemurro A1 - CANGIOTTI, A M A1 - RAGAZZO, V A1 - DI CICCO, M A1 - Vinci, B. A1 - G. Vozzi A1 - MACCHIA, P A1 - BONER, A L KW - Bioengineering VL - 64 ER - TY - JOUR T1 - Criticality of the Biological and Physical Stimuli Array Inducing Resident Cardiac Stem Cell Determination JF - STEM CELLS Y1 - 2008 A1 - G. Forte A1 - CAROTENUTO, F A1 - F. Pagliari A1 - S. Pagliari A1 - COSSA, P A1 - FIACCAVENTO, R A1 - A. Ahluwalia A1 - G. Vozzi A1 - Vinci, B. A1 - SERAFINO, A A1 - RINALDI, A A1 - E. Traversa A1 - CAROSELLA, L A1 - M. Minieri A1 - P. Di Nardo KW - Bioengineering VL - 26 ER - TY - JOUR T1 - HEMET: mathematical model of biochemical pathways for simulation and prediction of HEpatocyte METabolism JF - COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE Y1 - 2008 A1 - C. De Maria A1 - GRASSINI, D A1 - Vozzi, F. A1 - Vinci, B. A1 - A. Landi A1 - A. Ahluwalia A1 - G. Vozzi KW - Bioengineering VL - 92 ER - TY - JOUR T1 - In vitro engineering of the liver using multiple axis stimuli JF - INTERNAL MEDICINE Y1 - 2008 A1 - Vinci, B. A1 - G. Vozzi A1 - AVOGARO, A A1 - A. Ahluwalia KW - Bioengineering VL - 15 ER - TY - PAT T1 - MULTI-CAVITY BIOREACTOR CHAMBER Y1 - 2008 A1 - A. Ahluwalia A1 - D. Mazzei A1 - Vinci, B. KW - Bioengineering ER - TY - JOUR T1 - Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/poly(epsilon-caprolactone) blends for tissue engineering applications in the form of hollow fibers JF - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A Y1 - 2008 A1 - V. Chiono A1 - G. Ciardelli A1 - G. Vozzi A1 - SOTGIU, MG A1 - Vinci, B. A1 - C. Domenici A1 - P. Giusti KW - Bioengineering ER - TY - JOUR T1 - Rapid-prototyped and salt-leached PLGA scaffolds condition cell morpho-functional behaviour JF - JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A Y1 - 2008 A1 - Mattioli-Belmonte, M. A1 - G. Vozzi A1 - K KYRIAKIDOU A1 - PULIERI, E A1 - LUCARINI, G A1 - Vinci, B. A1 - PUGNALONI, A A1 - BIAGINI, G A1 - A. Ahluwalia KW - Bioengineering ER -